comparison between amperometric and chemiluminescence methods in detection and dosimetry of cobalt-60 gamma ray emission

background: hydrogen peroxide (h2o2), produced by gamma ray irradiation to watery solution, was used as an analytical parameter for dosimetry of cobalt-60 (60co) radiation. materials and methods: detection of the produced h2o2 was carried out using two methods: an amperometric biosensor fabricated by immobilization of anthraquinone 2-carboxylic acid modified horseradish peroxidase on glassy carbon electrode, and chemiluminescence technique using luminol as a fluorophore and diperiodatocuprate as a catalyst. results: in the first method, at the applied potential of -550 mv (vs. ag/agcl) the biosensor showed the sensitivity of 74.46 na/gray and detection limit of 0.061 gray (r.s.d=3.67% for n=3) towards h2o2 produced by 60co. in the second method, the sensitivity and detection limit for 60co gamma ray was determined to be 2.34 (luminescence intensity/gray) and 0.069 gray (r.s.d=4.16% for n=3), respectively. conclusion: the obtained results revealed that both proposed techniques, with leaner range from 0.25 to 5 grays are applicable for detection and dosimetry of gamma ray from 60co.